Biosurfactant production by a newly isolated soft coral-associated marine Bacillus sp.E34: Statistical optimization and characterization
نویسندگان
چکیده
Marine biosurfactant-producing bacteria were isolated from the soft coral Sarchophyton glaucum collected from Red sea. The main criteria used for screening of biosurfactant producers were haemolytic activity, dropcollapse, oil displacement and emulsification index. Based on phenotypic characterization and analysis of 16S rDNA sequencing the most potent isolate was identified as Bacillus sp.E34. To the best of our knowledge, this work is one of the early attempts to isolate and characterize biosurfactant-producing bacteria from soft coarls. Molasses enhanced biosurfactant production and maximum production occurred at 96 h incubation. The Plackett–Burman design was implemented to screen the medium components that significantly influence the production. The biosurfactant was stable over a wide range of pHs, temperatures and salinity, and had a good degree of emulsification with different hydrophobic substrates. Based on FT-IR analysis it was identified as glycolipopeptide. The biosurfactant is a potential candidate for bioremediation of hydrocarbon-contaminated sites as well as in other industrial applications. [Mona E. M. Mabrouk, Eman M. Youssif, Soraya A. Sabry. Biosurfactant production by a newly isolated soft coral-associated marine Bacillus sp.E34: Statistical optimization and characterization. Life Sci. J 2014; 11(10):756-768] (ISSN: 1097-8135). http://www.lifesciencesite.com. 123
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